Figure 5.
Immune function assessment. a-b) In vitro OVA-specific CD8+ T cell activation by α-AP-fmNP-loaded BMDCs. T cells collected from the OT-I mice were stained with CFSE and co-cultured with mature BMDCs loaded with PBS, APOVA, α-APOVA, or α-APOVA-fmNPs at a T cell: DC ratio of 50: 1. T cell proliferation was measured via CFSE fluorescence dilution using flow cytometry. c) Measurement of IFN-γ concentrations. d-e) Measurement of the killing activity of CTL. Data are presented as the mean ± SEM (n = 3).