Table 1.
Sequence information of the primers and probes for the ddPCR assays.
| Mutation | Primer and probe | Sequence |
|---|---|---|
| TERT (c.1-124C>T) | Forward primer | 5'-GTCCTGCCCCTTCACCTT-3' |
| Reverse primer | 5'-CAGCGCTGCCTGAAACTC-3' | |
| Mutant probe | 5'-FAM-AGCCCCCTCTGGG-MGB-3' | |
| Wild type probe | 5'-VIC-AGCCCCCTCCGGG-MGB-3' | |
| CTNNB1 (121A>G) | Forward primer | 5'-TCACTGGCAGCAACAGTCTT-3' |
| Reverse primer | 5'-CAGGACTTGGGAGGTATCCA-3' | |
| Mutant probe | 5'-FAM-GTGCCACTGCCAC-MGB-3' | |
| Wild type probe | 5'-VIC-GGTGCCACTACCAC-MGB-3' | |
| CTNNB1 (133 T>C) | Forward primer | 5'-TCACTGGCAGCAACAGTCTT-3' |
| Reverse primer | 5'-CAGGACTTGGGAGGTATCCA-3' | |
| Mutant probe | 5'-FAM-GTGCCACTGCCAC-MGB-3' | |
| Wild type probe | 5'-VIC-GGTGCCACTACCAC-MGB-3' | |
| TP53 (c.747G>T) | Forward primer | 5'-TGTAACAGTTCCTGCATGG-3' |
| Reverse primer | 5'-TCAGAGGCAAGCAGAGG-3' | |
| Mutant probe | 5'-FAM-CATGAACCGGAGTCCCATC-MGB-3' | |
| Wild type probe | 5'-VIC-CATGAACCGGAGGCCCATC-MGB-3' |