Figure 2.
Histological and Immunohistochemical Analysis of the large bowel during the progression of the model and experimental treatments. Samples were collected at the 6th day during the resting period after each DSS dose followed by paraffin inclusion and H&E staining. A, Healthy tissue where m=muscle and c=crypts; B, DSS-1; C, DSS-2; D, SSI or Colorectal Cancer; E, Doxorubicin 1 mg/Kg; F, Metformin - Sodium Oxamate (200-15) mg/Kg and G, Metformin - Sodium Oxamate - Doxorubicin (200-15-1) mg/Kg. 100X magnification. During the curse of the model, arrows shown slight crypt distortion, branched crypts in cross section as well as epithelial hyperplasia, reduction in globet cell numbers until adenoma with intramucosal carcinoma and haphazard proliferation of malignant cells within the lamina propria.