Skip to main content
. 2016 Jul 25;7(12):1653–1662. doi: 10.7150/jca.14762

Figure 4.

Figure 4

miR-34c suppressed GIT1 protein expression in MCF-7 cells. A. The transfection efficiency of miR-34c in MCF-7 cells was identified by qRT-PCR. B. Immunocytochemistry assay showed the GIT1 and Paxillin protein expression in cytoplasm of MCF-7 cells in different groups. Both GIT1 and Paxillin protein expression were suppressed by miR-34c transfection, and these effects were restored by AMO-miR-34c treatment in MCF-7 cells. C. Western blot detected GIT1 protein expression in MCF-7 cells of miR-34c, AMO-miR-34c and NC groups. D. The protein level of Paxillin in MCF-7 cells of miR-34c, AMO-miR-34c and NC groups detected by western blot technique. ** P<0.01 vs. NC group. *** P<0.001 vs. NC group. # P<0.05 vs. miR-34c group. ## P<0.01 vs. miR-34c group. n=3 independent batches of cells for each group.