Table 1.
Primers for qRT-PCR and luciferase assay.
| Primer | Primer Sequence (5'-3') | |
|---|---|---|
| mmu-miR-34c | RT | CTCAACTGGTGTCGTGGAGTCGGCAAGAGTCGGCAATTCAGTTGAGGCAATCAGCTAAC |
| Forward | ACACTCCAGCTGGGAGGCAGTGTAGTTAGCTGAT | |
| mmu-miR-93 | RT | CTCAACTGGTGTCGTGGAGTCGGCAAGAGTCGGCAATTCAGTTGAGCTACCTGCACG |
| Forward | ACACTCCAGCTGGGCAAAGTGCTGTTCGTGCAGG | |
| mmu-miR-21 | RT | CTCAACTGGTGTCGTGGAGTCGGCAAGAGTCGGCAATTCAGTTGAGTCAACATCAGTC |
| Forward | ACACTCCAGCTGGGTAGCTTATCAGACTGATGTT | |
| Mmu-miR-27a | RT | CTCAACTGGTGTCGTGGAGTCGGCAAGAGTCGGCAATTCAGTTGAGGCGGAACTTAG |
| Forward | ACACTCCAGCTGGG TTCACAGTGGCTAAGTTCC | |
| mmu-miR-375 | RT | CTCAACTGGTGTCGTGGAGTCGGCAAGAGTCGGCAATTCAGTTGAGTCACGCGAGCCG |
| Forward | ACACTCCAGCTGGGTTTGTTCGTTCGGCTCGCGT | |
| miRNA | Reverse | CTCAACTGGTGTCGTGGA |
| U6 | RT | AACGCTTCACGAATTTGCGT |
| Forward | CTCGCTTCGGCAGCACA | |
| Reverse | AACGCTTCACGAATTTGCGT | |
| GIT1 | Forward | GCCGACAGTGACTATGAGAACA |
| Reverse | CCCGCAACAGTTCCTGAAT | |
| 18S | Forward | CCTGGATACCGCAGCTAGGA |
| Reverse | GCGGCGCAATACGAATGCCCC | |
| GIT1-luc | Forward | (XhoI)ccgctcgagCCTCTCTCCCCACACCCTCACC |
| Reverse | (NotI)ataagaatgcggccgcTTAACAGCTCATGGTCACTTCTTTATTTT | |
| mutGIT1-luc | Forward | CGGGTAGCCAGTTTTGGTCCTGCCACGACGGAGCCTGATCCCTCCCCACTCTCGCCCC |
| Reverse | GGGGCGAGAGTGGGGAGGGATCAGGCTCCGTCGTGGCAGGACCAAAACTGGCTACCCG |
Note: miRNA Reverse primer as a common reverse primer used for all miRNAs detected by qRT-PCR in our study. XhoI and NotI are name of restriction enzyme.