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. 2016 Sep 28;6:33784. doi: 10.1038/srep33784

Figure 4. Oil Red O staining and gene expression of adipogenic differentiation markers 7 days after transfecting the C/EBPβ gene.

Figure 4

(a) Oil Red O staining of non-treated control cells cultured in growth media. (b) Oil Red O staining of non-treated control cells cultured in ADM. (c) Oil Red O staining of cells expressing the C/EBPβ gene transfected with Lipofectamine 2000 and cultured in ADM. (d) and (e) Oil Red O staining of cells expressing the C/EBPβ gene transfected with AuPEINPs (1.06 × 1011 and 1.33 × 1011 number of particles, respectively) and cultured in ADM. (f) and (g) Oil Red O staining of cells expressing the C/EBPβ gene transfected with AuMUAPEINPs (0.35 × 1011 and 0.53 × 1011 number of particles, respectively) and cultured in ADM. (h) Quantitative analysis of lipid droplet formation. (i–k) qRT-PCR analysis of the relative mRNA expressions of C/EBPβ, PPARγ2, and AP2 in cells transfected with the C/EBPβ gene and cultured in ADM. All values are expressed as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control non-transfected group. #p < 0.05, ##p < 0.01, and ###p < 0.001 versus Lipofectamine transfected group.