Figure 1.

MUC1 peptides (anchor-optimized and glycosylated) stabilized HLA-A*0201 molecules on T2 cells. Increasing amounts of the MUC1 peptides (0–100 μg/mL) and β2-microglobulin (1 μg/mL) were incubated with T2 cells for 18 h at 37 °C prior to staining with the HLA-A*0201 antibody (clone BB7.2, Pharmingen). Mean fluorescence intensity (MFI) was determined by flow cytometry and the fluorescence index (FI) was calculated from the formula: FI = (F_S − F_B)/(F_T2 − F_B) × 100 where F_S is the MFI of the test peptides, F_B is the non-peptide isotype antibody-stained control MFI, and F_T2 is the non-peptide HLA-A*0201 antibody-stained control MFI.