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CTLs were lytic to DCs pulsed with the immunizing peptide and showed cross-reactive lytic activity to the native P1 peptide STAPPVHNV. Autologous DCs pulsed with various MUC1 peptides (50 μg/mL) and PADRE peptide (10 μg/mL) were used as targets. Effector:target ratio was 100:1 and spontaneous release was less than 15% of complete lysis. * p < 0.05 (Student’s t-test) compared to the negative peptide.
