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. 2016 Aug 19;6(3):36. doi: 10.3390/biom6030036

Figure 2.

Figure 2

Internalization and processing of the Tau protein (15 µg; A–C,H) and its highly phosphorylated form (1.5 µg; D–F,I) by Neuro2A cells. Following 8 h (A,D), 24 h (B,E), or 48 h (C,F) of incubation with protein, cells were immunostained with a mouse anti-His-tag antibody targeted by an AlexaFluor® 594 antibody (red) and a rabbit anti-microtubule-associated protein 2 (MAP2) antibody targeted by an AlexaFluor® 488 antibody (green). In other experiments, cells were placed in contact with protein for 8 h, whereupon the medium was replaced by one devoid of protein and maintained for 48 h (H,I). The negative control (G) showed a non-specific distribution of the anti-His-tag antibody, localized only in the nucleus. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). All scale bars correspond to 10 µm.