Figure A3.

Determination of the minimal concentration needed to observe internalization of the low phosphorylated Tau protein inside cortical cells after 24 h of incubation. Initial protein amounts of 1.5 µg (A) and 4.5 µg (B) were used, but no signal was detected until 15 µg of the protein were added (Figure 2E and Figure 3E). Scale bars, 10 µm.