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P7 ethanol reduces MBP content. (A) 16 and 24 h after P7 saline/ethanol (EtOH) injection, MBP in the forebrain homogenates was analyzed using anti-MBP antibody (Santa Cruz) by Western blot; (B) 48 h after saline/ethanol injection at P7, mice were perfusion-fixed, and the brain sections were labeled using anti-MBP antibody. The representative images show the corpus callosum/cingulum regions. The bar indicates 50 µm.
