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. 2016 Jul 5;9(3):41. doi: 10.3390/ph9030041

Figure 7.

Figure 7

Enzymatic digestion of TSLP. (A) TSLP was digested with S. aureus V8 proteinase, and cleavage products were visualized by Western blot analysis using polyclonal antibodies against human TSLP. Products produced by V8 cleavage of TSLP revealed a major immunoreactive protein fragment at about 16 kDa. (B) TSLP and LL-37 were incubated with and without human neutrophil (leukocyte) elastase (HLE), S. aureus V8 proteinase or Pseudomonas aeruginosa elastase (PAE) and analyzed under non-reducing conditions by SDS-PAGE. From ref. [7].