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Schematic illustration of the biotin-capture assay for MurG activity [91]. In this assay, MurG catalyzes a reaction between radiolabeled (depicted by a yellow star) UDP-GlcNAc and biotinylated Lipid I analogue, and Lipid II with the radiolabel is synthesized. The avidin resin, which binds to biotin, therefore catches the radioactivity, which can be counted to indicate the MurG activity.
