. 2016 Aug 31;14(9):158. doi: 10.3390/md14090158

Table 2.

Primers selected for mRNA expression as target genes.

Gene	Name	Primer Sequence	Efficiency	R²	Anneling Temperature
VDAC	Voltage-dependent anion channel 1	F: CGGAAGGCAGAAGATGGC	101.6%	0.936	57 °C
VDAC	Voltage-dependent anion channel 1	R: TTGGTGGTCTCAGTGTTGG	101.6%	0.936	57 °C
SHMT2	Serine Hydroxymethyl transferase 2	F: CTGCGACTTCCGAGTTGCGATG	101.6%	0.963	57 °C
SHMT2	Serine Hydroxymethyl transferase 2	R: GGCTGCGTTGCTGTGCTGAG	101.6%	0.963	57 °C
TRAIL	Tumor Necrosis factor superfamily, member 10	F: TCTCTCTGTGTGGCTGTAAC	97.9%	0.992	57 °C
TRAIL	Tumor Necrosis factor superfamily, member 10	R: TCATACTCTCTTCGTCATTGGG	97.9%	0.992	57 °C
VIPR	Vasoactive Intestinal Peptide receptor 1	F: CACCATCAACTCCTCACTG	105.7%	0.992	57 °C
VIPR	Vasoactive Intestinal Peptide receptor 1	R: CTGCTGTCACTCTTCCTG	105.7%	0.992	57 °C