Figure 4.
Effect of LMWCs on the phosphorylation of IKBα in RAW264.7 macrophage. (A) Each cell population (4 × 105 cells/mL) was treated with LMWCs (3 kDa and 50 kDa) at the indicated concentrations of 40 μg/mL for 12 h, respectively (1: Control; 2: LPS; 3: 3 kDa chitosan; 4: 50 kDa chitosan); (B) Each cell population (4 × 105 cells/mL) was treated with LMWCs (3 kDa and 50 kDa) at the indicated concentrations of 40 μg/mL and LPS (1 μg/mL) for 12 h after pre-incubation with 20 μmol/L of wedelolactone (Wed) for 12 h, respectively (1: Control; 2: Wedelolactone (Wed); 3: Wed + LPS; 4: Wed + 3 kDa chitosan; 5: Wed + 50 kDa chitosan). The figures shown are representative of three independent experiments. These represent mean values of three independent experiments. Values are presented as means ± SD (n = 3). Bars with different letters (a, b, c, d) are statistically different (p < 0.05).