Table 1.
Real-Time PCR Primers and Conditions.
| Gene | Genbank Accession | Primer Sequence | Product Size (bp) | Annealing (°C) |
|---|---|---|---|---|
| COX2 | NM_011198.3 | TCTGGCTTCGGGAGCACAAC | 144 | 64 |
| GGTGTTGCACGTAGTCTTCGATCA | ||||
| IL-10 | NM_010548.2 | CAGTCGGCCAGAGCCACAT | 144 | 64 |
| CTTGGCAACCCAAGTAACCCTT | ||||
| MCP-1 | NM_011333.3 | CTGCATCTGCCCTAAGGTCTTCA | 101 | 64 |
| AGTGCTTGAGGTGGTTGTGGAA | ||||
| TRAF6 | D84655.1 | GGAGGACAAGGTTGCCGAAAT | 73 | 63 |
| CCCAAACTTGCCAATCTTCCAA | ||||
| JNK1 | NM_001310454.1 | GCTCTCCAGCACCCATACATCA | 109 | 63 |
| CCTCTATTGTGTGCTCCCTCTCAT | ||||
| IKKβ | AF088910.1 | CAGAAGTACACCGTGACCGTTGA | 104 | 63 |
| CACTGCACAGGCTGCCAGTTA | ||||
| 18S | NR_003278 | CGGACACGGACAGGATTGACA | 94 | 64 |
| CCAGACAAATCGCTCCACCAACTA |