Figure 2.

Sulforaphane and Protandim promote viability of OLN-93 cells under tert-butyl hydrogen peroxide-induced oxidative insult. OLN-93 cells were treated with 5 µM SFN, 90 µM MMF, 60 µg/mL Protandim or their respective DMSO or EtOH vehicle control for 24 h. After removal of medium, cells were subsequently exposed to either control medium or medium with 200 µM tert-butyl hydrogen peroxide. Cell viability was measured using an Invitrogen live/dead cytotoxicity kit. Data are presented as percentage of control and expressed as the mean ± SEM of 3 independent experiments. Statistics reflect one-way ANOVA test with post hoc Bonferroni correction; ** p < 0.01.