Table 1. Time constants of the isomerization reaction and enzymatic activity of CHT-DHI.
| Isomerization Reaction | Systems | Time Constant (sec) | |
|---|---|---|---|
| DHI (in acetonitrile) | CHT-DHI (in buffer) | ||
| Cis to trans | 4.8 | 7.6 | |
| Trans to cis | 53.3 | 342.9 | |
| Enzymatic Activity | Time Constant (min) | ||
| Dark | Light | ||
| CHT | 4.0 | 4.0 | |
| CHT-DHI | 100 | 5.0 | |
| D1 | L1 | ||
| Dark then Light | 100 | 4.8 | |
The same lamp source is used for all the light triggered processes. Moreover, the temperature is maintained at 25 °C.