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. 2016 Sep 28;6:34399. doi: 10.1038/srep34399

Table 1. Time constants of the isomerization reaction and enzymatic activity of CHT-DHI.

Isomerization Reaction Systems Time Constant (sec)
DHI (in acetonitrile) CHT-DHI (in buffer)
  Cis to trans 4.8 7.6
Trans to cis 53.3 342.9
Enzymatic Activity   Time Constant (min)
Dark Light
CHT 4.0 4.0
CHT-DHI 100 5.0
  D1 L1
Dark then Light 100 4.8

The same lamp source is used for all the light triggered processes. Moreover, the temperature is maintained at 25 °C.