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. 2016 Sep 28;6:34448. doi: 10.1038/srep34448

Figure 5. Enzyme activity in knockout and complemented parasite strains.

Figure 5

(a) Cellular aminopeptidase activity with different fluoropeptide substrates, including AAP-specific substrates H-Asp-MCA and H-Glu-MCA. Enzyme activity curves of whole protein lysates from various parasite strains are shown and were generated using GraphPad Prism v. 5.0c (GraphPad Software, San Diego, USA). Enzyme activities were recorded at 3 min intervals until maximum activity levels were reached. Samples devoid of protein lysates were used as negative controls. All experiments were independently repeated three times (each in triplicate), and representative curves are shown. (b) Activities in TgAAP-knockout parasites toward different substrates (x-axis) calculated as percentages of the activity in Cas9 control lysates (y-axis). Student’s t-test was used for calculation of significance between groups. *P < 0.05, **P < 0.01 (n = 3).