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. 2016 Sep 28;6:34448. doi: 10.1038/srep34448

Table 2. Kinetic parameters for the hydrolysis of peptide substrates by rTgAAP.

Substrate kcat (×10−2 s−1)a Km (μM)a kcat/Km (mM−1 s−1)
H-Glu-MCA 139.67 ± 30.65 7.35 ± 0.73 190.16
H-Asp-MCA 109.99 ± 6.48 12.84 ± 0.21 85.66
H-Asn-MCA 48.35 ± 1.26 24.32 ± 0.26 19.86
H-His-MCA 20.84 ± 0.43 88.70 ± 2.53 2.35
H-Ser-MCA 12.35 ± 0.86 92.87 ± 3.38 1.33
H-Pro-MCA 27.59 ± 2.37 98.55 ± 1.96 0.28

aData represent means ± SD from three independent experiments.