Table 2. Kinetic parameters for the hydrolysis of peptide substrates by rTgAAP.
Substrate | kcat (×10−2 s−1)a | Km (μM)a | kcat/Km (mM−1 s−1) |
---|---|---|---|
H-Glu-MCA | 139.67 ± 30.65 | 7.35 ± 0.73 | 190.16 |
H-Asp-MCA | 109.99 ± 6.48 | 12.84 ± 0.21 | 85.66 |
H-Asn-MCA | 48.35 ± 1.26 | 24.32 ± 0.26 | 19.86 |
H-His-MCA | 20.84 ± 0.43 | 88.70 ± 2.53 | 2.35 |
H-Ser-MCA | 12.35 ± 0.86 | 92.87 ± 3.38 | 1.33 |
H-Pro-MCA | 27.59 ± 2.37 | 98.55 ± 1.96 | 0.28 |
aData represent means ± SD from three independent experiments.