Figure 6. Docking model of the VSD_K-VSTx1 complex.

Residues implicated by the NMR data as being involved at the toxin-channel interface are highlighted in red (toxin residues) and orange (channel residues). VSD_K residues confirmed to be at the binding interface by mutagenesis are shown in red (E45/Q49/107), whilst peptide residues shown in red (R24/W25) are those that showed the greatest perturbations in NMR titrations with VSD_K. Orange residues are the remaining residues indicated by the titrations to be perturbed by complex formation. Hydrogen bonds are shown as yellow dotted lines. The helices are marked as S1-S4 in underlined font, while channel residues are in italics and peptide residues are in bold font. The C-terminus of the peptide is indicated near the S3-S4 loop.