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. 2016 Sep 28;6:34325. doi: 10.1038/srep34325

Figure 1. Whole-cell Kir4.1 currents recorded from cells expressing WT and R18Q channels.

Figure 1

Sample current traces for (a) WT and (b) R18Q channels expressed in U251 astrocytoma cells, obtained in response to voltage steps from −140 to +20 mV in 10 mV steps, from a holding potential of −30 mV. (c) Mean current density (pA/pF) as a function of voltage obtained from cells expressing the indicated channels. Inset: scatter plot of the single-cell current densities at −120 mV. The open symbols represent the mean ± SEM. (d) The resting membrane potentials were evaluated in current clamp conditions from cells expressing WT (black bar) or R18Q (grey bar) channels (data are mean ± SEM; **p < 0.01). (e) Sample inward-current densities evoked by puffs of an external solution containing 20 mM KCl from astrocytoma cells expressing the indicated channels (holding potential of −80 mV). (f) WT (black circles) and R18Q (grey circles) current densities elicited from each cell using the experimental protocol described in panel e which were subsequently averaged and depicted (mean ± SEM; *p < 0.05).