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. 2016 Sep 28;6:34312. doi: 10.1038/srep34312

Figure 4. Insulin-mediated enhancements in STAT3 signaling were mediated through GRP78 in SH-SY5Y-ObRb cells.

Figure 4

SH-SY5Y-ObRb cells were transfected with siRNA (5 nM) for 72 h. Seventy-two hours after the transfection, medium was changed to serum-free medium for another 20 h. Cells were treated with insulin (300 nM) for 4 h followed by leptin (0.03 μg/ml, 15 min). Phospho-STAT3 (Tyr705), STAT3, GRP78, and GAPDH levels were analyzed by Western blotting. (A) Leptin-induced STAT3 phosphorylation in insulin-treated cells was ameliorated in GRP78-knocked down cells. (B) A densitometric analysis of phospho-STAT3 (Tyr705) and GRP78 was performed using image analysis software. Data are expressed as the mean ± S.E. of 4 independent experiments (n = 4). **P < 0.01.