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. 2016 Sep 28;6:34427. doi: 10.1038/srep34427

Figure 1. Construction of binary vectors by fusing endogenous rice genes with TKTKK coding motifs.

Figure 1

(a) Construction of three binary vectors for improving lysine/threonine content. Top and middle panels showed the vectors pTKTKK1 and pTKTKK2, which were constructed by fusing a rice gene LOC_Os12g16880 or LOC_Os08g03579 with 16-fold TKTKK coding motifs TKTKKTKTKKKTKKKKKTKKKTKKKTKTKTRS, respectively. Bottom panel showed the binary vector pTKTKK3, which was constructed by tandom arraying both TKTKK1 and TKTKK2 in inverse order. (b,c) show the expression patterns of both genes LOC_Os12g16880 and LOC_Os08g03579, respectively. Expression data were achieved from NCBI GEO dataset with accession number GSE21396. Processed signal intensity, which was converted from processed raw data, was used to estimate the expression abundance in each gene among 11 tissues from different developmental stages. The prefix “LOC_” in each gene locus name was omitted for convenience. Tissue names in (b,c) were labelled in each column.