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. 2016 Jul 18;6(3):36. doi: 10.3390/bios6030036

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antibody conjugation analysis. (A) Analysis of antibody saturation study on the dithiobis succinimidyl propionate (DSP) functionalized Mo sensor. The dotted line indicates the noise threshold. Error bars indicate the standard error of mean from n = 3 replicates; (B) Baseline noise estimation on antibody conjugated biosensor and multiple PBS wash steps post the Ab conjugation. The impedance values for the phosphate-buffered saline (PBS) wash steps are statistically insignificant compared to the impedance obtained post the antibody incubation.