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. 2016 Sep 9;6(12):2052–2067. doi: 10.7150/thno.16180

Figure 6.

Figure 6

Th1/Th2 and Th17 immune responses influenced by NQDI 1 in a mouse model of crystalline silica-induced fibrosis. A Percentage of CD4+ IFN-γ+ Th1 cells in hilar lymph nodes was assayed by flow cytometry. B Relative expression of Th1 nuclear transcription factor T-bet in the lungs was assayed by real-time PCR. C Secretion of the Th1 cytokine IFN-γ in BALF was assayed by CBA. D Relative expression of Th2 nuclear transcription factor GATA-3 in the lungs was assayed by real-time PCR. E, F Secretion of Th2 cytokines IL-4 and IL13 in BALF was assayed by CBA and ELISA. G Percentage of CD4+ IL-17A+ Th17 cells in HLN was assayed by flow cytometry. H Relative expression of Th17 nuclear transcription factor ROR-γt in the lungs was assayed by real-time PCR. i Secretion of the Th17 cytokine IL-17A in BALF was assayed by CBA. For all experiments, n=5-6 per group per time point. # P<0.05 compared with the saline group; * P<0.05 compared with the crystalline silica group.