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. 2016 Sep 9;6(12):2052–2067. doi: 10.7150/thno.16180

Figure 8.

Figure 8

Blocking the 4-1BB signaling pathway reduced fibrocyte recruitment into the lungs. A Identification of lung fibrocytes. Gate P1 was set to gate out dead cells and crystalline silica particles. Gate P2 was set to collect CD45-positive cells from P1. Gate P3 was placed to circle the Col-1 positive cells from P2. Isotype rabbit IgG was used to identify the appropriate position of gate P3. B Percentage of CD45+ Col-1+ fibrocytes in the lungs. C, D Relative expression of ICAM-1 and VCAM-1 in the lungs was assayed by real-time PCR (n=5). E-H ELISA analysis of MCP-1 (CCL-2), MCP-5 (CCL-12), 6Ckine (CCL-21), and SDF-1α (CXCL-12) expression in lung tissues (n = 5-6). # P<0.05 compared with the saline group; * P<0.05 compared with the crystalline silica group.