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. 2016 Sep 28;6:34110. doi: 10.1038/srep34110

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Copyright © 2016, The Author(s)

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(A) Immunofluorescence staining for the spindle and chromosomes in MI oocytes after injection with GFP- (a,c–f) or Rad51- (b,g–j) dsRNA in GV oocytes that were then allowed to mature in vitro. The oocytes were fixed in 4% paraformaldehyde and then stained with an antibody against Pericentrin (red) and α-Tubulin (green). DNA was counterstained with DAPI (blue). Lower panel shows the magnified region of the spindle-chromosome complex in another MI oocyte after GFP RNAi (c–f) and Rad51 RNAi (g–j). The scale bars indicate 20 μm. (B) Proportion of GFP or Rad51-dsRNA injected MI oocytes with defects of spindle assembly and chromosome configuration. (C) RT-PCR analysis of the expression of MTOC-related genes, including Aurka, Aurkc, Bora, Cep192, Pcnt, Plk1 and Tacc3, in Rad51-silenced MI oocytes. H1foo was used as an internal control.