Table 2. Primer sequences and RT-PCR conditions.
| Gene symbol | Description | Accession numbers | Primer sequencesa | Annealing temperature | Product size |
|---|---|---|---|---|---|
| Rad51-Ab | RAD51 Recombinase | NM_011234.4 | For-AAGCTGCTTCAAGGTGGAAT | 60 °C | 507 bp |
| Rev-GAACATGGCTGCTCCATCTA | |||||
| Rad51-Bc | RAD51 Recombinase | NM_011234.4 | For- AGCTTTCAGCCAGGCAAAT | 60 °C | 265 bp |
| Rev-GCTTCAGCTTCAGGAAGACA | |||||
| Atg5 | Autophagy related 5 | NM_053069.5 | For- ATGTGCTTCGAGATGTGTGG | 60 °C | 215 bp |
| Rev- CAGGGGTGTGCCTTCATATT | |||||
| Atg7 | Autophagy related 7 | NM_001253717.1 | For-ATGCCAGGACACCCTGTGAACTTC | 60 °C | 351 bp |
| Rev-ACATCATTGCAGAAGTAGCAGCCA | |||||
| Atg12 | Autophagy related 12 | NM_026217.3 | For-TTCGGTTGCAGTTTCGCC | 63 °C | 311 bp |
| Rev-CCATGCCTGTGATTTGCAGTA | |||||
| Aurka | Aurora kinase A | NM_011497.4 | For-AGTTGGCAAACGCTCTGTCT | 60 °C | 160 bp |
| Rev-GTGCCACACATTGTGGTTCT | |||||
| Aurkc | Aurora kinase C | NM_001080965.1 | For-ACAACACCGGAACATCCTTC | 60 °C | 130 bp |
| Rev-TGCTGGTCCAACTTCTGATG | |||||
| Becn1 | Beclin1 | NM_019584.3 | For- TTTGACCATGCAATGGTAGC | 60 °C | 211 bp |
| Rev- TGGTCAGCATGAACTTGAGC | |||||
| Bora | Bora, aurora kinase A activator | NM_175265.4 | For-CCTGGTCCGCATACCTTTTA | 60 °C | 211 bp |
| Rev-CACGGTGTCATTTTCCCTCT | |||||
| Cep192 | Centrosomal protein 192kDa | NM_027556.1 | For-AGCTCAACTCGAGCCATCAT | 60 °C | 142 bp |
| Rev-GGGCTCAAAAACTTCAGTGC | |||||
| Map1lc3a | Microtubule-associated protein 1 light chain 3 alpha | NM_025735.3 | For-AGCTTCGCCGACCGCCGTAAG | 63 °C | 276 bp |
| Rev-CTTCTCCTGTTCATAGATGTCAGC | |||||
| Map1lc3b | Microtubule-associated protein 1 light chain 3 beta | NM_026160.4 | For-CGGAGCTTTGAACAAAGAGTG | 63 °C | 279 bp |
| Rev-TCTCTCACTCTCGTACACTTC | |||||
| Mtnd6 | Mitochondrially encoded NADH dehydrogenase 6 | NC_005089.1 | For-GAGGTTTAGGTTTAATTGTTAGTGGG | 60 °C | 175 bp |
| Rev- CAGTTAGATCCCCAAGTCTCTG | |||||
| Plk1 | Polo-like kinase 1 | NM_011121.3 | For-TGTAGTTTTGGAGCTCTGTCG | 60 °C | 148 bp |
| Rev-TCCCTGTGAATGACCTGATTG | |||||
| Pcnt | Pericentrin | NM_008787.3 | For- TGGAAAAGCTGTACCTGCAC | 60 °C | 192 bp |
| Rev- TGGTGAAGGGACGTGACATA | |||||
| Tacc3 | Transforming, acidic coiled-coil containing protein 3 | NM_001040435.3 | For-AAGTACAGCCAGAAAGACCTG | 60 °C | 299 bp |
| Rev-TCCTTCCGCTTCTCAAACC | |||||
| H1foo | H1 histone family, member O, oocyte-specific | NM_138311 | For- GCGAAACCGAAAGAGGTCAGAA | 60 °C | 378 bp |
| Rev- TGGAGGAGGTCTTGGGAAGTAA | |||||
| GFPb | Green fluorescent protein | KF111246.1 | For- TGTCCCAATTCTTGTTGAAT | 60 °C | 561 bp |
| Rev- TTGTCTGGTAAAAGGACAGG |
aFor, Forward; Rev, Reverse.
bPrimers were used for the preparation of Rad51 dsRNA and GFP dsRNA, respectively.
cDifferent set of primers was used to confirm the knockdown of Rad51 mRNA after RNAi treatment.