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. 2016 Sep 28;6:34281. doi: 10.1038/srep34281

Figure 2. Responsiveness to RA in the gonad during medaka development and correlation with aldh1a2 expression at stage 33.

Figure 2

Responsiveness to retinoic acid (Green) in embryonic stages 30 (A–C) 35 (D) and 37 (E) of double transgenic embryos (12XRARE-ef1::gfp::Vasa::H2B::mCherry). Germ cells, vasa positive (Red), at the gonad level. Responsiveness in the germ cells is apparent at stage 30 and switches to the nephric duct at later stages. In situ hybridization for alh1a2 transcripts show expression in the gonadal region in male (F) and female (G) in transversal section, and lateral view (H). Lateral view of double transgenic embryos (12XRARE-ef1::gfp (Green):: sox9b::mCherry (Red)) (I) showing no co-localization at this stage. An, anterior region of the embryo; Go, gonadal region; ND, nephric duct; Ps, posterior region of the embryo.