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. 2016 Sep 28;6:34083. doi: 10.1038/srep34083

Figure 4. MvfR binds to and fine tunes the expression of LasR QS system genes.

Figure 4

(a) ChIPseq analysis reveals that MvfR binds to rsaL-lasR region. The black bar above the binding intensity plots represents the peak identified using SPP peak caller. (b) RNAseq analysis indicates that MvfR induces the expression lasR, lasI and rsaL. Light blue bar = mvfR mutant at OD600nm 2, dark blue bar = mvfR mutant at OD600nm 3. (c) qRTPCR analysis validates that MvfR induces the expression of lasR. Faint blue bar = mvfR mutant at OD600nm 1, light blue bar = mvfR mutant at OD600nm 2. Data show the average +/− SEM of 3 independent replicates. Statistical significance was assessed using one way ANOVA + Dunnett’s post-test.