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. 2016 Sep 28;6:34083. doi: 10.1038/srep34083

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Copyright © 2016, The Author(s)

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(a,d) ChIPseq analysis reveals that MvfR binds to hsiA2-hcpD (T6SS HSI-II) and xcpQ-xcpP-xcpR (T2SS) regions. The black bar above the binding intensity plots represents the peak identified using SPP peak caller. (b,e) RNAseq analysis indicates that MvfR induces the expression of most T6SS and T2SS genes. Light blue bar = mvfR mutant at OD_600nm 2, dark blue bar = mvfR mutant at OD_600nm 3. (c,f) qRTPCR analysis validates that MvfR induces the expression of T6SS and T2SS genes. Faint blue bar = mvfR mutant at OD_600nm 1, light blue bar = mvfR mutant at OD_600nm 2. Data show the average +/− SEM of 3 independent replicates. Statistical significance was assessed using one way ANOVA + Dunnett’s post-test.