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. 2016 Sep 28;6:34022. doi: 10.1038/srep34022

Figure 5. FAIM2 inhibits the Fas-apoptosis pathway and inhibition of FAIM2 expression significantly decreases cell viability in response to Fas and etoposide treatment in SCLC cells.

Figure 5

(A) Western blot analysis of FAIM2, Fas and caspase-8 in SCLC and NSCLC cells. An inverse correlation between FAIM2 and proapoptotic proteins, Fas and Caspase-8 expression was observed. FAIM2 was significantly expressed in all three SCLC cell lines while only one NSCLC cell line (H1703) showed a clear FAIM2 expression. There was a very clear inverse correlation between FAIM2 and Fas and a modest correlation between FAIM2 and Caspase-8. Beta-actin used as an internal control. (B) We tested three siRNAs targeting FAIM2 and selected the most efficient one (siFAIM2-1) in the knockdown of FAIM2 expression (left panel). Cell viability was measured in the presence of Fas agonist to induce apoptosis after the transfection of FAIM2 siRNA. Cell viability was significantly reduced by FAIM2 inhibition (p < 0.05) (right panel). (C) Etoposide treatment synergistically reduced cell viability in SCLC cells (H128 and H69) with FAIM2 knockdown compared to that in non-silencing control (p < 0.05).