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. 2016 Sep 28;6:33972. doi: 10.1038/srep33972

Figure 2.

Figure 2

(a) Pull-down assay showing interaction of in vitro expressed TaMdm2 and Tap53. (b) Pull-down assay showing interaction of TaMdm2 and Tap53 expressed in H1299 cells. Tap53 detected using anti-FLAG antibody, p53 detected using DO1 antibody. (c) Disruption of the in vitro Tap53Δ-MDM2 interaction by the stapled peptide antagonists PM2 and MO11 (left panel). Disruption of Tap53Δ-TaMdm2 interaction by the stapled peptide antagonist MO11 (right panel). (d) Pull-down assay showing interactions of in vitro expressed Tap53 and Tap53M26L with MDM2 and TaMdm2. Control lanes (CON) show amounts of indicated p53 proteins pulled down in absence of MDM2/TaMdm2 immobilisation on beads. The calculated molecular weights of the indicated proteins are 56.3 KDa (MDM2, C-terminal HA tag), 43.7 KDa (p53), 39.5 KDa (p53Δ), 53 KDa (TaMdm2, C-terminal HA tag), 66.2 KDa (Tap53, C-terminal FLAG tag), 49.1 KDa (Tap53Δ, C-terminal FLAG tag). See Supplementary Information for uncropped blots.