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. 2016 Sep 7;5(3):36. doi: 10.3390/plants5030036

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Chromatographic purification steps of B. napus cruciferin from pH 8.5, 50 mM Tris-HCl extraction. (A) Chromatogram of protein extract separated on Sephadex G-25 Hiprep™ 26/10 desalting column; (B) Chromatogram of Peak 1 of desalting column separated on Resource S Cation Exchange Column (CEX); (C) Chromatogram of Peak 2 separated on Sephacryl S-300 Hiprep™ 26/10 high-resolution size exclusion column (SEC); (D) Polypeptide profiles of B. napus meal and Peak 1 obtained from desalting column; (E) Polypeptide profiles of Peak 2, Peak 3 and (F) Peak 4 obtained from CEC and S-300 SEC, respectively. Polypeptide profiles were obtained under non-reducing conditions using 8%–25% precast gradient gels. Each lane contained approximately the same protein content. MWM: Molecular weight markers.