Fig. 4.

Methylation analysis of Tol2, a 4682-bp long autonomous transposon, in medaka. The new genome assembly of SMRT reads had 17 regions (contigs) that contained complete Tol2 copies. The circles show our prediction of the methylation state of CpG sites, while the rectangles show the methylation states within each 100 bp window obtained from bisulfite sequencing. For both tracks, open indicates unmethylation and filled indicates methylation. The arrow above indicates the region of Tol2 insertions. As the eleventh 11th region was located at the extreme of the contig, Tol2 was not observed successfully by either SMRT sequencing or bisulfite sequencing. For the other 16 regions, methylation of Tol2 was observed consistently by SMRT sequencing, while virtually no information was available on the Tol2 region from bisulfite sequencing (Color version of this figure is available at Bioinformatics online.)