. 2016 Oct 5;129(19):2338–2346. doi: 10.4103/0366-6999.190666

Table 1.

Details for the primers for the target and reference genes used in quantitative real-time PCR

Gene	Primers	Primer Sequence	bp
GAPDH	tuzi-GAPDH-F	CGAGACACGATGGTGAAGGT	131
GAPDH	tuzi-GAPDH-R	ATGTAGTGGAGGTCAATGAATGG	131
Tu-COL2A1	Tu-COL2A1-F	ATGGCGGCTTCCACTTCA	92
Tu-COL2A1	Tu-COL2A1-R	CTCAGTGGACAGCAGGCG	92
Tu-agg	Tu-agg-F	TTACCACCTACCCTTCACCTG	90
Tu-agg	Tu-agg-R	TTCTTCTGTCCAAAGGTCCTG	90

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; COL2A1: Collagen Type II alpha 1; agg: Aggrecan; F: Forward; R: Reverse; PCR: Polymerase chain reaction.