Table 2.

Collagen Type II alpha 1 IHC staining intensity of the NP during organ culture

Culture time point	Controls	Static load	t	P
Day 0	47.64 ± 8.33	43.75 ± 7.05	0.798	0.448
Day 3	44.74 ± 3.26	61.54 ± 5.91*‡	−2.593	0.035
Day 7	43.47 ± 3.88	22.97 ± 5.03*†‡	2.470	0.039
Day 14	44.58 ± 5.02	30.43 ± 2.13*†‡	5.811	<0.001
F	0.198	20.417	–	–
P	0.896	0.000	–	–

Values are given as the mean ± SD. *The significance of differences between culture time point and fresh (day 0); ^†The significance of differences between culture time point and day 3; ^‡The significance of differences between static load and controls. IHC: Immunohistochemistry; NP: Nucleus pulposus; SD: Standard deviation, –: Not applicable.