Table 3.

Agg gene expression quantified by real-time PCR during organ culture

Culture time point	Controls	Static load	t	P
Day 0	1.000 ± 0.025	1.000 ± 0.037	−0.100	0.925
Day 3	1.088 ± 0.026	0.053 ± 0.060*‡	7.941	0.015
Day 7	0.101 ± 0.007*†	0.007 ± 0.001*†‡	24.012	0.002
Day 14	0.162 ± 0.009*†	0.027 ± 0.005*‡	21.788	0.001
F	63.048	1.903	–	–
P	0.000	0.000	–	–

Values are given as the mean ± SD. *The significance of differences between culture time point and fresh (day 0); ^†The significance of differences between culture time point and day 3; ^‡The significance of differences between static load and controls. Agg: Aggrecan; PCR: Polymerase chain reaction; SD: Standard deviation, –: Not applicable.