Table 4.
COL2A1 gene expression quantified by real-time PCR during organ culture
| Culture time point | Controls | Static load | t | P | |
|---|---|---|---|---|---|
| Day 0 | 1.000 ± 0.041 | 1.000 ± 0.038 | 0.933 | 0.404 | |
| Day 3 | 0.506 ± 0.042* | 1.195 ± 0.040*,‡ | −20.365 | 0.000 | |
| Day 7 | 0.368 ± 0.058* | 0.016 ± 0.002*,†,‡ | 3.946 | 0.017 | |
| Day 14 | 0.523 ± 0.028* | 0.005 ± 0.001*,†,‡ | 10.493 | 0.000 | |
| F | 14.020 | 1.561 | – | – | |
| P | 0.000 | 0.000 | – | – | |
Values are given as the mean ± SD. *The significance of differences between culture time point and fresh (day 0); †The significance of differences between culture time point and day 3; ‡The significance of differences between static load and controls. COL2A1: Collagen Type II alpha 1; PCR: Polymerase chain reaction; SD: Standard deviation; –: Not applicable.