Table 1.
IC50, LD50, and SI values obtained from in vitro tests with extracts (RG and RM), 1 obtained from toads venom, and chloroquine (CQ) against P. falciparum W2 strain
| Samples | IC50 |
LD50a |
SIa |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Value (μg/ml) | R | MTT |
NR |
MTT |
NR |
|||||
| HepG2 | BGM | HepG2 | BGM | HepG2 | BGM | HepG2 | BGM | |||
| RG | 0.050 | 0.5247 | 109.91 | 34.83 | > 100 | 22.62 | > 2,000 | > 500 | > 100 | > 100 |
| RM | 0.534 | 0.9396 | 57.00 | 38.90 | > 200 | > 200 | 72.84 | > 100 | > 100 | > 100 |
| 1 | 1.280 | 0.9646 | 102.16 | 133.86 | > 200 | > 200 | 84.50 | > 100 | > 100 | > 100 |
| CQb | 0.234 | 0.9894 | > 1,000 | > 1,000 | > 1,000 | > 1,000 | > 1,000 | > 1,000 | > 1,000 | > 1,000 |
a
LD50 and SI values were obtained with MTT and neutral red (NR) cytotoxic tests in human hepatoma cells (HepG2) and normal kidney glomerular cells (BGM).
b
Chloroquine: antimalarial used as a positive control at a concentration of 2-2,000 μg/ml.