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. 2016 Sep 27;7(5):e00796-16. doi: 10.1128/mBio.00796-16

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Copyright © 2016 DePas et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — MiPACT-HCR allows visualization of bacteria in cleared sputum samples. (a) Cartoon depicting the process of embedding and clearing sputum for visualization of bacteria via HCR. (b) The clearing process for sputum sample 5.1. Each grid square represents 1 mm². (c) Blend projections of five sputum samples after staining with DAPI (blue) and WGA (orange) from Z-stacks acquired with a 10× objective. (d) HCR with a universal bacterial probe (green; EUB338 with B1 hairpins conjugated to AlexaFluor 647) in sputum sample 5.1. The middle panel is a maximum intensity projection acquired with a 10× objective, and the right panel is a single-plane image acquired with a 25× objective. White arrows indicate PMNs.