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. 2016 Sep 20;7(5):e01515-16. doi: 10.1128/mBio.01515-16

FIG 1 .

FIG 1 

Two common lab strains, CEA10 and AF293, show phenotypic variation and strikingly different virulence in a triamcinolone model of IPA but not a leukopenic model of IPA. (A) Radial growth of CEA10 and AF293 on GMM in normoxia (~21% O2) and hypoxia (0.2% O2) at 96 h. (B) Biomass from liquid cultures grown in 1% glucose minimal medium (GMM) in normoxia and hypoxia at 48 h. ***, P = 0.0004; n.s., not significant by unpaired, two-tailed t test. (C) Ratio of biomass in hypoxia to biomass in normoxia, as calculated with values from panel B. *, P = 0.0165 by unpaired, two-tailed t test. (D) Survival analysis of CEA10 (n = 20 from 2 independent experiments) and AF293 (n = 27 from 3 independent experiments) in a triamcinolone model of IPA in CD-1 mice inoculated with 2 × 106 conidia intranasally. **, P = 0.0002 by log rank test. (E) Survival analysis of CEA10 (n = 16) and AF293 (n = 17) in a chemotherapeutic model of IPA in CD-1 mice inoculated with 2 × 106 conidia intranasally. n.s., not significant by log rank test. (F and G) Hematoxylin and eosin (H&E) or Gomori methenamine silver (GMS) stains of lungs 3 days postinoculation from triamcinolone-treated mice (F) and chemotherapy-treated leukopenic mice (G) inoculated with 2 × 106 AF293 or CEA10 conidia. Images are representative of three mice, and all error bars indicate standard errors of the means.