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. 2016 Sep 8;5:2269. [Version 1] doi: 10.12688/f1000research.8156.1

Figure 5. Treatment of neuronal cultures with IGF-1 rescues the small neuronal phenotype.

Figure 5.

Primary hippocampal cultures from Mecp2 A140V male animals were treated with IGF-1 (100 ng/ml) for 24 hrs on 3 DIV. Neurons were stained with anti-beta-III Tubulin (Tuj-1), and cross-sectional area of neurons was measured. Representative confocal images were obtained with 20 X objective. Immunofluorescence image showing ( A) untreated neurons and B) treated neurons with IGF-1. C) Quantification of neuronal soma size of mutant neurons without and with IGF-1 treatment revealed rescue of neuronal soma in the mutant neurons. (Mean ± SEM, *, p <0.05, Student's t-test).