Fig. 2. Cyclic voltammetry (CV) measurements at 5 mV s–1 of (A) Native Escherichia coli hydrogenase-1, and (B) the E73Q variant under gas atmospheres of both 3% H2 in N2 (dotted lines) and 0% H2 (100% N2, solid lines). Other experimental conditions: pH 4.5; 37 °C; electrode rotation rate 3500 rpm; carrier gas N2 and total gas flow rate 100 scc min–1. (C) The difference in H2 production activity is emphasised through an overlay of the averaged forward and back scan sweeps of the CVs in the top two panels, the selected region is highlighted by the dotted grey boxes in A and B.
