Figure 2.

Analysis of the expression status of UPR chaperones as a consequence of hepatic gene transfer of coF8 in C57BL/6 mice 4 weeks post treatment. (a) Western Blotting of liver tissue lysates for detection of BiP and (b) of CHOP in animals receiving 1 × 10¹⁰ vg (n = 5), 1 × 10¹¹ vg (n = 3), or 1 × 10¹² vg (n = 4) of AAV8-coF8 or 1 × 10¹² vg of AAV8 null vector (n = 5). For additional comparison, protein extract from a mouse treated with tunicamycin (T), that was untreated (N, “naive”), or that received 1 × 10¹² vg of AAV8-F9 vector (F9). AAV, adeno-associated virus; BiP, binding immunoglobulin protein; CHOP, CCAAT/enhancer-binding protein homologous protein; UPR, unfolded protein response.