| Buffer B (1L) | |
|---|---|
| - 30 mM MOPS | (6.28g) |
| - 15 mM MgCl2 | (3.05g) |
| - 25 mM KCl | (1.86g) |
| - 5 mM β-mercaptoethanol | (352μl/L of buffer) |
| - pH 8.0 | |
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