| Elution Buffer (100mL) | |
|---|---|
| - 30 mM MOPS | (628 mg) |
| - 15 mM MgCl2 | (305 mg) |
| - 25 mM KCl | (186 mg) |
| - 1 mM cAMP | (33 mg) |
| - 5 mM β-mercaptoethanol | (35.2μl/100 mL of buffer) |
| - pH 8.0 | |
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