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. 2016 Sep 23;22:3383–3393. doi: 10.12659/MSM.897301

Figure 1.

Figure 1

miR-483 is down-regulated in ovary cortex of PCOS patients and inhibits KGN cell viability and proliferation. pre-miR-483, KGN cells transfected with pre-miR-483. miR-483 sponge, KGN cells transfected with miR-483 sponge. pre/sponge control, KGN cells transfected with the corresponding blank vector as a control. (A) miR-483 expression in lesion and the adjacent normal ovary cortex from 20 PCOS patients as detected by qRT-PCR. (B) miR-483 expression was successfully altered by transfecting pre-miR-483 or miR-483 sponge in KGN cells. (C) Cell viability detected by MTT method at 24, 48, and 72 h after transfection. (D) Pictures of colony formation influenced by miR-483. (E) Colony formation rate calculated from colony formation assay. * P<0.05. ** P<0.01. *** P<0.001. PCOS, polycystic ovarian syndrome.