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. 2016 Sep 23;22:3383–3393. doi: 10.12659/MSM.897301

Figure 2.

Figure 2

miR-483 induces cell cycle arrest in KGN cells. pre-miR-483, KGN cells transfected with pre-miR-483. miR-483 sponge, KGN cells transfected with miR-483 sponge. pre/sponge control, KGN cells transfected with the corresponding blank vector as a control. (A) Cell cycle analyzed by flow cytometry at 24 h after transfection. (B) Average cell percent in G1/G0, S, and G2/M stages. (C) mRNA levels of cell cycle factors CCNB1, CCND1, and CDK2 detected by qRT-PCR with GAPDH as an internal reference. (D) Protein levels of CCNB1, CCND1 and CDK2 detected by Western blot with GAPDH as an internal reference. * P<0.05. ** P<0.01. CCNB1 – cyclin B1. CCND1 – cyclin D1. CDK2 – cyclin-dependent kinase 2.